Product Info Summary
SKU: | A00812-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Human CD163 DyLight® 488 conjugated Antibody
SKU/Catalog Number
A00812-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD163 DyLight® 488 conjugated Antibody catalog # A00812-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD163 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00812-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD163 recombinant protein (Position: T47-E201).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00812-Dyl488 is reactive to CD163 in Human
Applications
A00812-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
125.451kDa
Background of CD163
CD163 (Cluster of Differentiation 163) is a protein that in humans is encoded by the CD163 gene. The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For CD163 (Source: Uniprot.org, NCBI)
Gene Name
CD163
Full Name
Scavenger receptor cysteine-rich type 1 protein M130
Weight
125.451kDa
Alternative Names
CD163 antigenscavenger receptor cysteine-rich type 1 protein M130; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; M130macrophage-associated antigen; MM130; RM3/1 CD163 M130, MM130, SCARI1 CD163 molecule scavenger receptor cysteine-rich type 1 protein M130|hemoglobin scavenger receptor|macrophage-associated antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD163, check out the CD163 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD163: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD163 DyLight® 488 conjugated Antibody (A00812-Dyl488)
Hello CJ!
A00812-Dyl488 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
il-17 induces macrophages to M2-like phenotype via nF-%u03BAB
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Human CD163 DyLight® 488 conjugated Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Human CD163 DyLight® 488 conjugated Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Human CD163 DyLight® 488 conjugated Antibody
Question
Our lab were content with the WB result of your anti-Human CD163 DyLight® 488 conjugated antibody. However we have observed positive staining in plasma soluble cd163: secreted using this antibody. Is that expected? Could you tell me where is CD163 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
From literature, plasma does express CD163. Generally CD163 expresses in soluble cd163: secreted, cell membrane. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-14
Question
We are currently using anti-Human CD163 DyLight® 488 conjugated antibody A00812-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
The anti-Human CD163 DyLight® 488 conjugated antibody (A00812-Dyl488) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-06
Question
We have observed staining in human plasma. Any tips? Is anti-Human CD163 DyLight® 488 conjugated antibody supposed to stain plasma positively?
H. Johnson
Verified customer
Asked: 2017-01-31
Answer
From what I have seen in literature plasma does express CD163. From what I have seen in Uniprot.org, CD163 is expressed in esophagus, spleen, plasma, liver, among other tissues. Regarding which tissues have CD163 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218, 24275569
Plasma, Pubmed ID: 16335952
Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-01-31